A fruiting body tissue method for efficient Agrobacterium-mediated transformation of Agaricus bisporus.
نویسندگان
چکیده
We describe a modified Agrobacterium-mediated method for the efficient transformation of Agaricus bisporus. Salient features of this procedure include cocultivation of Agrobacterium and fruiting body gill tissue and use of a vector with a homologous promoter. This method offers new prospects for the genetic manipulation of this commercially important mushroom species.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 66 10 شماره
صفحات -
تاریخ انتشار 2000